Maize rbcS promoter activity depends on sequence elements not found in dicot rbcS promoters.

rbcS SRS4 promoter from Glycine max and its expression activity in transgenic tobacco.

The regulatory region of the ribulose-1,5-bisphosphate carboxylase small subunit gene SRS4 from soybean (Glycine max) was cloned using TAIL-PCR and general PCR, and named the rbcS promoter. The promoter was fused with the GUS gene and introduced into Nicotiana tabacum via Agrobacterium-mediated leaf disk transformation. In 4-week-old transgenic tobacco plants, the highest GUS expression levels ...

The ABCs of RBCs

Test Release Processes | RBCS

In the last issue, I introduced this series of articles on critical software testing processes. For our first article, let’s look at the processes associated with an essential precondition for any test activities: creation, delivery, and installation of a software release into the test environment. Without software to test, there can be no software testing, but, as obvious as this is, many soft...

The Pea rbcS-3A Promoter Mediates Light Responsiveness but not Organ Specificity.

The expression of the pea rbcS-3A gene is responsive to light and restricted to chloroplast-containing cells. Previously, we identified transcriptional elements between -410 and -50 relative to the transcription start site as being sufficient for this regulated expression. Here we investigate the role of the promoter region (-50 to +15) in rbcS-3A expression. We demonstrate an interaction betwe...

Effect of the Polydispersity of RBCs on the Recovery Rate of RBCs during the Removal of CPAs

In the process of removing cryoprotectants from cryopreserved blood, the theoretically optimal operating condition, which is based on the assumption that the distribution of red blood cells is uniform, is often used to reduce or even avoid the hypotonic damage to cells. However, due to the polydispersity of cells, the optimal condition is actually not reliable. In this study, based on the discr...